Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA.
In the absence of an effective vaccine (s), the control of African swine fever is caused by African swine fever virus (ASFV) should be based on early, efficient, cost-effective detection and control and elimination of strict strategy. To this end, we developed an indirect ELISA was able to detect antibodies ASFV either serum or oral fluid specimens. Recombinant proteins used in the ELISA selected by comparing the serum antibody responses beginning of ASFV-infected pigs (NHV-P68 isolates) Fruit fly Recombinant Proteins for the three major recombinant polypeptide (p30, P54, p72) using multiplex fluorescent microbead-based immunoassay (FMIA). Not dangerous serum (non-infectious) antibody-positive to be used as a positive control plates and for the calculation of sample-to-positive (S: P) ratio was produced by inoculation of pigs with replicon particle vaccines (RP) revealed that ASFV p30 gene. Optimized ELISA detected anti-p30 antibodies in serum and / or oral fluid samp...