Cloning, tissue distribution, expression pattern, and function of porcine maternal embryonic leucine zipper kinase.
Background Mrs. embryonic leucine zipper kinase (Melk) is an atypical member of snf1 / AMPK family of serine-threonine kinases, are involved in physiological and pathological processes vary, including cell proliferation, apoptosis, embryogenesis , cancer Mouse Recombinant Proteins treatment resistance, and RNA processing. Melk is highly expressed in human cancers and is associated with a more aggressive form of astrocytoma, glioblastoma, breast cancer, and melanoma until now, no information about swine Melk (pMELK) have been reported. method In this study, pMELK coding sequence was cloned from pig spleen and characterized. We also quantitatively determined the expression of Melk in 11 isolated from pig tissue and subcellular localization is determined as stated in the swine umbilical vein endothelial cells (SUVEC) as a fusion protein. In addition, we report the functional characterization of proteins pMELK regarding its role in apoptosis. sequencing analysis showed that the full-l...