Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs.
In recent years, many studies have shown that a live recombinant adenovirus vector-based vaccine is a promising new vaccine candidates against viral infection. Therefore, in this study, a new type of recombinant adenovirus expressing spike (S) protein Cavia Recombinant Proteins pig epidemic diarrhea virus (PEDV), RAD-PEDV-S, produced, and characteristics are determined. Then, its efficacy as a vaccine candidate was evaluated in pigs 4-week-old. The results showed that protein S can also be expressed at high levels in the RAD-PEDV-S-infected cells and that the viral titers can reach 1 011 PFU / mL. Furthermore, the results of animal experiments show that the RAD-PEDV-S pose a significant response specific PEDV humoral immunity after vaccination (P <0.05). In addition, RAD-PEDV-S provides partial protection for swine against PEDV extremely lethal challenge. The results presented in this study demonstrate that the adenovirus vector can be used as a vaccine vector for vacc...