Skip to main content

Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs.

In recent years, many studies have shown that a live recombinant adenovirus vector-based vaccine is a promising new vaccine candidates against viral infection. Therefore, in this study, a new type of recombinant adenovirus expressing spike (S) protein Cavia Recombinant Proteins pig epidemic diarrhea virus (PEDV), RAD-PEDV-S, produced, and characteristics are determined. 

Then, its efficacy as a vaccine candidate was evaluated in pigs 4-week-old. The results showed that protein S can also be expressed at high levels in the RAD-PEDV-S-infected cells and that the viral titers can reach 1 011 PFU / mL. Furthermore, the results of animal experiments show that the RAD-PEDV-S pose a significant response specific PEDV humoral immunity after vaccination (P <0.05). In addition, RAD-PEDV-S provides partial protection for swine against PEDV extremely lethal challenge. 

The results presented in this study demonstrate that the adenovirus vector can be used as a vaccine vector for vaccine development PEDV and a promising new vaccine candidates for the prevention and control of swine epidemic diarrhea (PED) future, but its efficacy still needs to be improved in the future.
Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs.

Construction and immunological evaluation of a recombinant vaccine expressing Newcastle disease virus highly pathogenic pig reproductive and respiratory syndrome virus GP3 / GP5 protein in pigs.


Highly pathogenic pig reproductive and respiratory syndrome (HP-PRRS) is a significant threat to the pig industry, the vaccination is considered an effective means of prevention and control. Here, we developed two Newcastle disease virus (NDV) Lasota-PRRS vaccine candidate recombinant vectors, rLaSota-GP5 and rLaSota-GP3-GP5, using reverse genetic engineering. 

Both recombinant virus exhibited a high degree of genetic stability after 10 generations in a row in chicken embryos. There are no significant differences in pathogenicity as compared to the parent strain rLaSota in birds, rats and pigs. Recombinant virus can Chicken Recombinant Proteins not be detected in immunized pigs eating environment, but can be detected in the organs and tissues of pigs no more than 10 days after immunization. 

What is important, in contrast with rLaSota-GP5, rLaSota-GP3-GP5 raises both humoral and cellular immune responses are significant in pigs. In particular, neutralizing antibody titers in the group rLaSota-GP3-GP5 is 1.51 times higher than the commercial vaccine group at 42 days post-immunization. At the same time, there are significant differences in the levels of IFN-γ between groups rLaSota-GP3-GP5 and commercial vaccine group
Labels:

Comments

Post a Comment

Popular posts from this blog

Cloning, tissue distribution, expression pattern, and function of porcine maternal embryonic leucine zipper kinase.

Background Mrs. embryonic leucine zipper kinase (Melk) is an atypical member of snf1 / AMPK family of serine-threonine kinases, are involved in physiological and pathological processes vary, including cell proliferation, apoptosis, embryogenesis , cancer Mouse Recombinant Proteins  treatment resistance, and RNA processing. Melk is highly expressed in human cancers and is associated with a more aggressive form of astrocytoma, glioblastoma, breast cancer, and melanoma until now, no information about swine Melk (pMELK) have been reported. method In this study, pMELK coding sequence was cloned from pig spleen and characterized. We also quantitatively determined the expression of Melk in 11 isolated from pig tissue and subcellular localization is determined as stated in the swine umbilical vein endothelial cells (SUVEC) as a fusion protein. In addition, we report the functional characterization of proteins pMELK regarding its role in apoptosis. sequencing analysis showed that the full-l...
Post a Comment
Read more

Development of Recombinant Protein-Based Vaccine Against Classical Swine Fever Virus in Pigs Using Transgenic Nicotiana benthamiana.

Classical swine fever virus (CSFV) is highly contagious and fatal to infected pigs. Vaccines against CSFV have been developed from inactivated or modified live virus. The vaccine is effective for immunization of animals, but they are associated with E.coli Recombinant Proteins  problems such as accidental spread of animal viruses in the field, and with trade barriers following vaccination. Here, we report the generation of transgenic Nicotiana benthamiana plants for large-scale, cost-effective production E2 fusion proteins for use as a recombinant vaccine against CSFV in pigs. Transgenic N. benthamiana plants save an intergenic, single-copy insertion of a chimeric gene encoding a fusion protein E2 has a high level of expression of the transgene. For large-scale production of the fusion protein E2 leaf tissue, we developed protein purification protocol consisting of a cellulose-binding domain (CBD) -cellulose affinity based purification and size-exclusion gel filtration chromatogr...
Post a Comment
Read more

Detection of African Swine Fever Virus Antibodies in Serum and Oral Fluid Specimens Using a Recombinant Protein 30 (p30) Dual Matrix Indirect ELISA.

In the absence of an effective vaccine (s), the control of African swine fever is caused by African swine fever virus (ASFV) should be based on early, efficient, cost-effective detection and control and elimination of strict strategy. To this end, we developed an indirect ELISA was able to detect antibodies ASFV either serum or oral fluid specimens.  Recombinant proteins used in the ELISA selected by comparing the serum antibody responses beginning of ASFV-infected pigs (NHV-P68 isolates) Fruit fly Recombinant Proteins  for the three major recombinant polypeptide (p30, P54, p72) using multiplex fluorescent microbead-based immunoassay (FMIA). Not dangerous serum (non-infectious) antibody-positive to be used as a positive control plates and for the calculation of sample-to-positive (S: P) ratio was produced by inoculation of pigs with replicon particle vaccines (RP) revealed that ASFV p30 gene.  Optimized ELISA detected anti-p30 antibodies in serum and / or oral fluid samp...
Post a Comment
Read more